The transcription network of pluripotency

Cellular identity depends on gene regulatory networks governed by transcription factors. Discovering these for the pluripotent state is important for its applications.

Diagram of the rudimentary transcription network of mouse embryonic stem cells.

Brightfield image of human embryonic stem cells cultured in a feeder-free naïve pluripotent state.


Human naïve pluripotency

Human embryonic stem cells are traditionally cultured in the primed state, resembling the post-implantation epiblast. The development of methods to keep human pluripotent stem cells in the naïve state enabled access to an earlier stage of human development, equivalent to the pre-implantation inner cell mass.


The cell cycle on the exit from pluripotency

The exit from pluripotency is a prerequisite to the many applications of human embryonic stem cells that require their differentiation. This process is tightly linked to the cell cycle, with the G1 phase permissive to the exit from pluripotency, and the S and G2 phases actively opposing it.

Fluorescence image montage of human embryonic stem cells in various treatment conditions. The green fluorescent protein reporter is driven by the promoter of NANOG, a pluripotent gene. Nuclei are stained with Hoechst 33342 in blue.

Early liver organoid with hepatocytes fluorescently labelled by ALB immunostaining in red and cholangiocytes labelled by CK7 immunostaining in green.


Stem cell-derived organoids

Organoids are self-organized three-dimensional cultures that replicate many complex aspects of an in vivo organ. As such, they serve as a unique in vitro platform for complex disease modeling and drug discovery.

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